Aim of this research was to evaluate the hepatoprotective potential of different fractions P. dicoccos (Rubiaceae) in wistar rats against CCl4 intoxicated liver damage. Rats were acclimatized for 7 days, after those rats were divided into six groups, each group consisting of six animals. Group I: Served as a control. Group II: Treated with vehicle daily for 15 days followed by CCl4 on the 2nd and 3rd. Group III (Silymarin): Animals treated 50 mg/kg of Silymarin for 15 days orally followed by CCl4 an 2nd and 3rd. Group IV, V and VI: treated in similar way using mthanolic, petroleum ether and water extract of 300 mg/kg, respectively followed by CCl4 an 2nd and 3rd. The preliminary phytochemical investigation of crude extracts of P. dicoccos leaves revealed the presence of pytoconstituents, which were identified as phenols, flavonoids, Saponins terpenoids proteins, carbohydrates alkaloids. The activities of serum AST, ALT, ALP and TB levels were significantly (P < 0.001) increased with a significant decrease in total protein and albumin levels in toxic group as compared to the control and standard group. The levels of the enzymes were significantly reversed on treatment with P. dicoccos in a dose-dependent manner. The degree of protection was observed maximally with the test group 300 mg/kg. The water extract showed a better hepatoprotective activity (P<0.001) than methanolic as well as petroleum ether fractions.
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